| 1. | Agarose gel electrophoresis
琼脂糖凝胶电泳 |
| 2. | Agarose gel electrophoresis video clip wmv format , window media player required
琼脂糖凝胶电泳示范片段wmv格式，需用window media player播放 |
| 3. | Dna ladder bands with a periodicity of about 200 bp were clearly seen in agarose gel electrophoresis pattern
琼脂糖凝胶电泳基因组dna可见到约以200bp为间隔的dna梯状( ladder )条带。 |
| 4. | Apoptotic peak . agarose gel electrophoresis showed that 185 - base pair ladder characteristic of the dna degradation that occurs in apoptotic cells induced by a
电泳出现阶梯状条带流式细胞仪检测出现典型的凋亡峰，提示 |
| 5. | The result of the agarose gel electrophoresis showed that the length of the full - length cdnas in the library was pooled mainly between 500 and 2 000 base pairs
结果表明获得的ejoi基因的cdna长度为876hp ，开放阅读框长度为759hp ，编码252个氨基酸。 |
| 6. | 0 . 5 % agarose gel electrophoresis and eb staining demonstrated that the " tail " of dna strand was the longest when being treated with 1 mmol / lmms , which illustrate the severest of dna - damage
Fl浓度时dna条带“拖尾”最长，说明损伤最重。同时mms明显提高了各组细胞的端粒酶活性，甚至在相对较低浓度( 0 |
| 7. | 2 ) screening using pcr . sequencing of pgex - hbrp the plasmid was screened by pcr , and 1 % agarose gel electrophoresis . the results show that there was one band at the respected sites about 750bp
2 ) pcr鉴定经过双酶切鉴定的质粒dna用pcr扩增，结果可见，在750bp处出现明显的带，表明插人的片段确是外源 |
| 8. | 2 . cloning of the pcr products the pcr products were purified by agarose gel electrophoresis and was ligated with pucm - t vector . by the method of pcr and enzyme digest analysis . the result shows that the plasmid containing cpti gene was transferred into e . coli dhso
Pcr产物的克隆采用a / t克隆法，将pcr产物经琼脂糖凝胶电泳纯化回收后用t4连接酶与pucm - t载体连接，构建成克隆载体puc - cp ，转化大肠杆菌dh _ 5 。 |
| 9. | The high purity of genomic dna extracted by tripure isolation reagent was observed . dna agarose gel electrophoresis showed that the genome had a high integrity without degradation . and also , spectrophotometric analysis indicated that the genomic dna had no pollution by protein and rna . 2
培养乳酸乳球菌nizor5 ，收集菌体，用tripurelsolationreagent提取其基因组dna ，琼脂糖凝胶电泳，紫外显示仪下可见在点样孔附近有一条整齐均一的dna带。 |
| 10. | The plasmid was tested by the restriction enzyme bamh i and xho i incision , and 1 % agarose gel electrophoresis . the results show that there were two bands at the respected sites about 4 . 9kb and 750bp respectively . it means that hbrp has been cloned into a pgex - 5x - 1 expression vector correctly
2 .质粒鉴定pgex一hbrp融合蛋白表达质粒转化eoh . bi21感受态细胞l )限制性内切酶鉴定提取质粒dna ，经bamhfxhol双酶切鉴定，结果可见，有750bp的插人片段和4 . gkb的质粒dna两条带，表明所提质粒中含有外源基因hbrp片段，大小及插人方向均正确。 |
